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ATCC e coli mg1655

Growth curves and intracellular thiol analysis <t>E.</t> <t>coli</t> ATCC 25922 growth curves and intracellular thiol analysis in the presence of compounds 3d (red), 3l (dark yellow), and 3n (green) at the static MICs (A and B) and at 3-fold static MICs (C and D). Cultures grown in the presence of 1% DMSO (white) and 5 mM (345 μg/mL) 1,2,4-triazole (gray) were included as negative and positive controls, respectively. The bars represent the average of at least 3 biological replicates, and the error bars represent the SD. The statistical significance of thiol decrease was determined by a two-way ANOVA test (see ). ∗ p < 0.1; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

E Coli Mg1655, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Benzotriazole derivatives as alternate O- acetylserine sulfhydrylase substrates to impair cysteine biosynthesis in gram-negative bacteria"

Article Title: Benzotriazole derivatives as alternate O- acetylserine sulfhydrylase substrates to impair cysteine biosynthesis in gram-negative bacteria

Journal: iScience

doi: 10.1016/j.isci.2025.113818

Growth curves and intracellular thiol analysis E. coli ATCC 25922 growth curves and intracellular thiol analysis in the presence of compounds 3d (red), 3l (dark yellow), and 3n (green) at the static MICs (A and B) and at 3-fold static MICs (C and D). Cultures grown in the presence of 1% DMSO (white) and 5 mM (345 μg/mL) 1,2,4-triazole (gray) were included as negative and positive controls, respectively. The bars represent the average of at least 3 biological replicates, and the error bars represent the SD. The statistical significance of thiol decrease was determined by a two-way ANOVA test (see ). ∗ p < 0.1; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Figure Legend Snippet: Growth curves and intracellular thiol analysis E. coli ATCC 25922 growth curves and intracellular thiol analysis in the presence of compounds 3d (red), 3l (dark yellow), and 3n (green) at the static MICs (A and B) and at 3-fold static MICs (C and D). Cultures grown in the presence of 1% DMSO (white) and 5 mM (345 μg/mL) 1,2,4-triazole (gray) were included as negative and positive controls, respectively. The bars represent the average of at least 3 biological replicates, and the error bars represent the SD. The statistical significance of thiol decrease was determined by a two-way ANOVA test (see ). ∗ p < 0.1; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Techniques Used:

Fractional inhibitory concentration (FIC) index of the best combinations among 3d , 3l , and 3n BT derivatives with different antibiotics Antibiotic-BT derivatives were tested on E. coli ATCC 25922 (magenta), S. Typhimurium ATCC 14028 (violet), and K. pneumoniae ATCC 13883 (light blue). The data represent the FIC index between colistin, gentamicin, ciprofloxacin, or ampicillin and the most effective concentration of BT derivative. An FIC index ≤0.5 is indicative of synergy of action, 0.5 < FIC ≤1 shows additivity of action, and 1 < FIC <4 shows indifference between the couples of considered compounds. The complete set of data is reported in .

Figure Legend Snippet: Fractional inhibitory concentration (FIC) index of the best combinations among 3d , 3l , and 3n BT derivatives with different antibiotics Antibiotic-BT derivatives were tested on E. coli ATCC 25922 (magenta), S. Typhimurium ATCC 14028 (violet), and K. pneumoniae ATCC 13883 (light blue). The data represent the FIC index between colistin, gentamicin, ciprofloxacin, or ampicillin and the most effective concentration of BT derivative. An FIC index ≤0.5 is indicative of synergy of action, 0.5 < FIC ≤1 shows additivity of action, and 1 < FIC <4 shows indifference between the couples of considered compounds. The complete set of data is reported in .

Techniques Used: Concentration Assay

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Journal: iScience

Article Title: Benzotriazole derivatives as alternate O- acetylserine sulfhydrylase substrates to impair cysteine biosynthesis in gram-negative bacteria

doi: 10.1016/j.isci.2025.113818

Figure Lengend Snippet: Growth curves and intracellular thiol analysis E. coli ATCC 25922 growth curves and intracellular thiol analysis in the presence of compounds 3d (red), 3l (dark yellow), and 3n (green) at the static MICs (A and B) and at 3-fold static MICs (C and D). Cultures grown in the presence of 1% DMSO (white) and 5 mM (345 μg/mL) 1,2,4-triazole (gray) were included as negative and positive controls, respectively. The bars represent the average of at least 3 biological replicates, and the error bars represent the SD. The statistical significance of thiol decrease was determined by a two-way ANOVA test (see ). ∗ p < 0.1; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: E. coli MG1655 , ATCC , N/A.

Techniques:

Journal: iScience

Article Title: Benzotriazole derivatives as alternate O- acetylserine sulfhydrylase substrates to impair cysteine biosynthesis in gram-negative bacteria

doi: 10.1016/j.isci.2025.113818

Figure Lengend Snippet: Fractional inhibitory concentration (FIC) index of the best combinations among 3d , 3l , and 3n BT derivatives with different antibiotics Antibiotic-BT derivatives were tested on E. coli ATCC 25922 (magenta), S. Typhimurium ATCC 14028 (violet), and K. pneumoniae ATCC 13883 (light blue). The data represent the FIC index between colistin, gentamicin, ciprofloxacin, or ampicillin and the most effective concentration of BT derivative. An FIC index ≤0.5 is indicative of synergy of action, 0.5 < FIC ≤1 shows additivity of action, and 1 < FIC <4 shows indifference between the couples of considered compounds. The complete set of data is reported in .

Article Snippet: E. coli MG1655 , ATCC , N/A.

Techniques: Concentration Assay

Effects of varied ZnCl 2 concentrations on growth kinetics parameters for Escherichia coli grown under aerobic (labeled with +O 2 ) and anaerobic (labeled with –O 2 ) conditions . A – C , growth parameters for aerobically grown E. coli , ( D – F ) growth parameters for anaerobically grown E. coli. Lag time, growth rate, and max OD 600 represent the mean ± SEM of three biological replicates for each condition and Zn 2+ concentration. Growth parameters were calculated from growth curves ( <xref ref-type=

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: Effects of varied ZnCl 2 concentrations on growth kinetics parameters for Escherichia coli grown under aerobic (labeled with +O 2 ) and anaerobic (labeled with –O 2 ) conditions . A – C , growth parameters for aerobically grown E. coli , ( D – F ) growth parameters for anaerobically grown E. coli. Lag time, growth rate, and max OD 600 represent the mean ± SEM of three biological replicates for each condition and Zn 2+ concentration. Growth parameters were calculated from growth curves ( Fig. S1 , A and B ) using nonlinear regression curve fitting to a four-parameter Logistic equation with GraphPad Prism 9 software. NS, not significant; ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗ ∗∗∗p ≤ 0.0001 as determined by one-way ANOVA with Tukey multiple comparison test.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Labeling, Concentration Assay, Software, Comparison

ICP-MS quantification of metal contents of Escherichia coli grown aerobically (+O 2 ) and anaerobically (–O 2 ) in LB and LB containing 0.5 mM added ZnCl 2 . Data are shown as individual data points overlaid on the bar representation, with error bars as SD from three biological replicates. NS, not significant; ∗p ≤ 0.05; ∗∗∗p ≤ 0.001 as determined by one-way ANOVA with Tukey multiple comparison test.

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: ICP-MS quantification of metal contents of Escherichia coli grown aerobically (+O 2 ) and anaerobically (–O 2 ) in LB and LB containing 0.5 mM added ZnCl 2 . Data are shown as individual data points overlaid on the bar representation, with error bars as SD from three biological replicates. NS, not significant; ∗p ≤ 0.05; ∗∗∗p ≤ 0.001 as determined by one-way ANOVA with Tukey multiple comparison test.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Comparison

Intracellular zinc in aerobic live Escherichia coli cells detected by fluorescent protein based Zn 2+ sensors . ( A ) CreiLOV N41C , ( B ) ZapCY2, and ( C ) ZapCY1. The fluorescence emission of CreiLOV N41C was recorded initially and every 30 s after addition of TPEN (50 μM) and ZnCl 2 /saponin/pyrithione (100 μM/0.002%/1.5 μM). The fluorescence emission of ZapCY2 and ZapCY1 was recorded initially and every 1 min after addition of TPEN (5 μM) and ZnCl 2 /saponin/pyrithione (10 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2 and ZapCY1). All error bars represent the standard deviation for three biological replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: Intracellular zinc in aerobic live Escherichia coli cells detected by fluorescent protein based Zn 2+ sensors . ( A ) CreiLOV N41C , ( B ) ZapCY2, and ( C ) ZapCY1. The fluorescence emission of CreiLOV N41C was recorded initially and every 30 s after addition of TPEN (50 μM) and ZnCl 2 /saponin/pyrithione (100 μM/0.002%/1.5 μM). The fluorescence emission of ZapCY2 and ZapCY1 was recorded initially and every 1 min after addition of TPEN (5 μM) and ZnCl 2 /saponin/pyrithione (10 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2 and ZapCY1). All error bars represent the standard deviation for three biological replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Fluorescence, Standard Deviation

Fluorescence microscopy of Escherichia coli expressing CreiLOV N41C under aerobic and anaerobic conditions. A , aerobically grown and imaged E. coli . B , anaerobically grown and imaged E. coli. From left to right in A and B : initial fluorescence signal from CreiLOV N41C , fluorescence signal 5 min after the addition of 50 μM TPEN, 15 min after the addition of 100 μM ZnCl 2 /0.002% saponin/1.5 μM pyrithione, and 20 min after the addition of 200 μM TPEN. The scale bar represents 10 μm. C and D , quantification of change in fluorescence signals shown in parts A and B , respectively. Mean cell intensities were normalized to the initial fluorescence intensity. Individual data points representing individual cells are overlaid on the bar chart representation and were collected from three biological replicates, each with two or more technical replicates. Data were analyzed by means of one-way ANOVA with Tukey multiple comparison test. ∗∗∗∗ p < 0.0001. Error bars represent the standard deviation. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine.

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: Fluorescence microscopy of Escherichia coli expressing CreiLOV N41C under aerobic and anaerobic conditions. A , aerobically grown and imaged E. coli . B , anaerobically grown and imaged E. coli. From left to right in A and B : initial fluorescence signal from CreiLOV N41C , fluorescence signal 5 min after the addition of 50 μM TPEN, 15 min after the addition of 100 μM ZnCl 2 /0.002% saponin/1.5 μM pyrithione, and 20 min after the addition of 200 μM TPEN. The scale bar represents 10 μm. C and D , quantification of change in fluorescence signals shown in parts A and B , respectively. Mean cell intensities were normalized to the initial fluorescence intensity. Individual data points representing individual cells are overlaid on the bar chart representation and were collected from three biological replicates, each with two or more technical replicates. Data were analyzed by means of one-way ANOVA with Tukey multiple comparison test. ∗∗∗∗ p < 0.0001. Error bars represent the standard deviation. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Fluorescence, Microscopy, Expressing, Comparison, Standard Deviation

Endogenous zinc as detected by fluorescent protein-based Zn 2+ sensors in live cell suspensions of Escherichia coli grown aerobically and anaerobically under different protein expression conditions . Detection of endogenous Zn 2+ by CreiLOV N41C when protein expression was induced with 0.5 mM IPTG for 3 h at 37 °C and cells were grown ( A ) aerobically or ( B ) anaerobically. Detection of endogenous Zn 2+ by ( C ) CreiLOV N41C , ( D ) ZapCY2, and ( E ) ZapCY1 when protein expression was induced by 0.1 mM IPTG at 18 °C overnight and cells were grown aerobically. Under all conditions, E. coli cells were grown in the presence of ZnCl 2 (0–2 mM). For each sample, the fluorescence emission was recorded initially and every 30 s or 1 min after addition of TPEN (50 μM) and ZnCl 2 /saponin/pyrithione (100 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2 and ZapCY1). All error bars represent the standard deviation for 3 biological replicates, each with ≥2 technical replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: Endogenous zinc as detected by fluorescent protein-based Zn 2+ sensors in live cell suspensions of Escherichia coli grown aerobically and anaerobically under different protein expression conditions . Detection of endogenous Zn 2+ by CreiLOV N41C when protein expression was induced with 0.5 mM IPTG for 3 h at 37 °C and cells were grown ( A ) aerobically or ( B ) anaerobically. Detection of endogenous Zn 2+ by ( C ) CreiLOV N41C , ( D ) ZapCY2, and ( E ) ZapCY1 when protein expression was induced by 0.1 mM IPTG at 18 °C overnight and cells were grown aerobically. Under all conditions, E. coli cells were grown in the presence of ZnCl 2 (0–2 mM). For each sample, the fluorescence emission was recorded initially and every 30 s or 1 min after addition of TPEN (50 μM) and ZnCl 2 /saponin/pyrithione (100 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2 and ZapCY1). All error bars represent the standard deviation for 3 biological replicates, each with ≥2 technical replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Expressing, Fluorescence, Standard Deviation

Zn 2+ ion influx in live cell suspensions of Escherichia coli grown aerobically and anaerobically as monitored by fluorescent protein-based Zn 2+ sensors. Zn 2+ ion influx for E. coli grown aerobically ( A and B ) and anaerobically ( C ), detected by CreiLOV N41C ( A and C ) and ZapCY2 ( B ). For each sample, the fluorescence emission was recorded initially and every 10 min after addition of ZnCl 2 (0–75 μM), TPEN (100 μM for CreiLOV N41C and 150 μM for ZapCY2) and ZnCl 2 /saponin/pyrithione (200 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2). All error bars represent the standard deviation for three biological replicates, each with ≥2 technical replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Journal: The Journal of Biological Chemistry

Article Title: Fluorescent protein-based Zn 2+ sensors reveal distinct responses of aerobic and anaerobic Escherichia coli cultures to excess Zn 2+

doi: 10.1016/j.jbc.2024.107840

Figure Lengend Snippet: Zn 2+ ion influx in live cell suspensions of Escherichia coli grown aerobically and anaerobically as monitored by fluorescent protein-based Zn 2+ sensors. Zn 2+ ion influx for E. coli grown aerobically ( A and B ) and anaerobically ( C ), detected by CreiLOV N41C ( A and C ) and ZapCY2 ( B ). For each sample, the fluorescence emission was recorded initially and every 10 min after addition of ZnCl 2 (0–75 μM), TPEN (100 μM for CreiLOV N41C and 150 μM for ZapCY2) and ZnCl 2 /saponin/pyrithione (200 μM/0.002%/1.5 μM). Buffer: 50 mM HEPES, 100 mM NaCl, pH 7.1. λ ex = 450 nm (CreiLOV N41C ) and 433 nm (ZapCY2). All error bars represent the standard deviation for three biological replicates, each with ≥2 technical replicates. TPEN, N,N,N′,N′ -tetrakis-(2-pyridylmethyl)-ethylenediamine. HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid.

Article Snippet: E. coli strain MG1655 was from American Type Culture Collection and made chemically competent by CaCl 2 .

Techniques: Fluorescence, Standard Deviation

Comparative transcriptome analysis of E. coli chassis cells for metabolic utilization of ethylene glycol. Whole transcriptome sequencing (RNA-seq) was performed to quantify gene expression in EG02 under two distinct conditions: LB liquid medium and M9 (10 g/L EG) medium. The analysis results are presented in the proposed pathways for ethylene glycol metabolism in E. coli . The gray dotted box provides an explanation of the data presentation style legend. FPKM (fragments per kilobase of exon model per million reads) indicates the expression level of each gene. The log 2 fold change represents the fold increase in gene expression of EG02 in M9 (10 g/L EG) medium compared to LB medium. The solid black box compares the production of reducing equivalents when using glucose and ethylene glycol as sole carbon sources. fucO, lactaldehyde reductase; aldA, aldehyde dehydrogenase A; glcDEF, glycolate dehydrogenase; glcB, malate synthase G; gcl, glyoxylate carboligase; hyi, hydroxypyruvate isomerase; glxK, glycerate 2-kinase 2; glxR, tartronate semialdehyde reductase 2. EG, Ethylene glycol; GLA, glycolaldehyde; GA, Glycolate; GLO, Glyoxylate; TSA, (2R)-tartronate semialdehyde; (OH)-PYR, hydroxypyruvate; GLR, D-glycerate; 2PG, 2-phospho-D-glycerate; MAL, malate

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock

doi: 10.1186/s13068-024-02568-4

Figure Lengend Snippet: Comparative transcriptome analysis of E. coli chassis cells for metabolic utilization of ethylene glycol. Whole transcriptome sequencing (RNA-seq) was performed to quantify gene expression in EG02 under two distinct conditions: LB liquid medium and M9 (10 g/L EG) medium. The analysis results are presented in the proposed pathways for ethylene glycol metabolism in E. coli . The gray dotted box provides an explanation of the data presentation style legend. FPKM (fragments per kilobase of exon model per million reads) indicates the expression level of each gene. The log 2 fold change represents the fold increase in gene expression of EG02 in M9 (10 g/L EG) medium compared to LB medium. The solid black box compares the production of reducing equivalents when using glucose and ethylene glycol as sole carbon sources. fucO, lactaldehyde reductase; aldA, aldehyde dehydrogenase A; glcDEF, glycolate dehydrogenase; glcB, malate synthase G; gcl, glyoxylate carboligase; hyi, hydroxypyruvate isomerase; glxK, glycerate 2-kinase 2; glxR, tartronate semialdehyde reductase 2. EG, Ethylene glycol; GLA, glycolaldehyde; GA, Glycolate; GLO, Glyoxylate; TSA, (2R)-tartronate semialdehyde; (OH)-PYR, hydroxypyruvate; GLR, D-glycerate; 2PG, 2-phospho-D-glycerate; MAL, malate

Article Snippet: The E. coli strains MG1655, ATCC 8739, DH5α, and BL21(DE3) underwent genetic modification to enable ethylene glycol metabolism.

Techniques: Sequencing, RNA Sequencing, Gene Expression, Expressing

Characterization of ethylene glycol metabolism in E. coli MG1655 and UV-induced mutant E. coli . In LB medium, both EG01 (green) and E. coli MG1655 (blue) exhibited doubling times of 1.58 h −1 and 1.26 h −1 , respectively. In M9 medium with ethylene glycol as the sole carbon source, EG01 (black) had a doubling time of 5.22 h. −1 , whereas the growth of E. coli MG1655, MG1655 ( fucO ) overexpressing fucO , and MG1655 ( fucO / aldA ) overexpressing both fucO and aldA was undetectable. The red line illustrates the change in ethylene glycol consumption by EG01 in M9 (10 g/L EG) medium. Error bars indicated standard error ( n = 3)

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock

doi: 10.1186/s13068-024-02568-4

Figure Lengend Snippet: Characterization of ethylene glycol metabolism in E. coli MG1655 and UV-induced mutant E. coli . In LB medium, both EG01 (green) and E. coli MG1655 (blue) exhibited doubling times of 1.58 h −1 and 1.26 h −1 , respectively. In M9 medium with ethylene glycol as the sole carbon source, EG01 (black) had a doubling time of 5.22 h. −1 , whereas the growth of E. coli MG1655, MG1655 ( fucO ) overexpressing fucO , and MG1655 ( fucO / aldA ) overexpressing both fucO and aldA was undetectable. The red line illustrates the change in ethylene glycol consumption by EG01 in M9 (10 g/L EG) medium. Error bars indicated standard error ( n = 3)

Article Snippet: The E. coli strains MG1655, ATCC 8739, DH5α, and BL21(DE3) underwent genetic modification to enable ethylene glycol metabolism.

Techniques: Mutagenesis

Enhancing E. coli chassis cells for ethylene glycol as a non-sugar feedstock. EG01 underwent continuous cultivation for 24 days, with transfers to fresh medium every two days, covering a span of 12 generations, resulting in the strain EG02. Compared to EG01, after 48 h of cultivation in M9 (10 g/L EG) medium, the final biomass increased from 0.2 ± 0.1 OD to 2.1 ± 0.6 OD, and the uptake rate of ethylene glycol (uptake rate of EG) also rose from 4.8 ± 0.8 mmol/gDW.h to 8.1 ± 1.3 mmol/gDW.h. Error bars indicated standard error ( n = 3)

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock

doi: 10.1186/s13068-024-02568-4

Figure Lengend Snippet: Enhancing E. coli chassis cells for ethylene glycol as a non-sugar feedstock. EG01 underwent continuous cultivation for 24 days, with transfers to fresh medium every two days, covering a span of 12 generations, resulting in the strain EG02. Compared to EG01, after 48 h of cultivation in M9 (10 g/L EG) medium, the final biomass increased from 0.2 ± 0.1 OD to 2.1 ± 0.6 OD, and the uptake rate of ethylene glycol (uptake rate of EG) also rose from 4.8 ± 0.8 mmol/gDW.h to 8.1 ± 1.3 mmol/gDW.h. Error bars indicated standard error ( n = 3)

Article Snippet: The E. coli strains MG1655, ATCC 8739, DH5α, and BL21(DE3) underwent genetic modification to enable ethylene glycol metabolism.

Techniques:

Rational engineering of E. coli chassis cells utilizing ethylene glycol as the sole carbon source. A Schematic diagram illustrating plasmid construction for the pathways enabling ethylene glycol metabolism. Module I: PM93- fucO / aldA ; Module II: PM93- glcD / glcE / glcF ; Module III: PM93- gcl / hyi / glxK / glxR ; Module IV: PM93- glcB . B Reverse metabolic engineering of E. coli MG1655 for ethylene glycol utilization. Individual or co-transformation of Module I, II, III, and IV plasmids into E. coli MG1655 strains. The OD 600 value of the strain after 72 h is shown in the blue bar graph, while the residual percentage of ethylene glycol (%) is depicted in the red pie chart. C Rational engineering of six E. coli chassis cells ( E. coli MG1655, ATCC8739, E. coli DH5α, E. coli BL21(DE3), EG01, and EG02) utilizing ethylene glycol as the sole carbon source. The OD 600 value of the strains after 72 h is represented in the blue bar graph, while the residual amount of ethylene glycol (g/L) is shown in the red bar graph. M9 (10 g/L EG) medium was employed for culturing all E. coli strains mentioned above. Error bars indicate standard error ( n = 3)

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock

doi: 10.1186/s13068-024-02568-4

Figure Lengend Snippet: Rational engineering of E. coli chassis cells utilizing ethylene glycol as the sole carbon source. A Schematic diagram illustrating plasmid construction for the pathways enabling ethylene glycol metabolism. Module I: PM93- fucO / aldA ; Module II: PM93- glcD / glcE / glcF ; Module III: PM93- gcl / hyi / glxK / glxR ; Module IV: PM93- glcB . B Reverse metabolic engineering of E. coli MG1655 for ethylene glycol utilization. Individual or co-transformation of Module I, II, III, and IV plasmids into E. coli MG1655 strains. The OD 600 value of the strain after 72 h is shown in the blue bar graph, while the residual percentage of ethylene glycol (%) is depicted in the red pie chart. C Rational engineering of six E. coli chassis cells ( E. coli MG1655, ATCC8739, E. coli DH5α, E. coli BL21(DE3), EG01, and EG02) utilizing ethylene glycol as the sole carbon source. The OD 600 value of the strains after 72 h is represented in the blue bar graph, while the residual amount of ethylene glycol (g/L) is shown in the red bar graph. M9 (10 g/L EG) medium was employed for culturing all E. coli strains mentioned above. Error bars indicate standard error ( n = 3)

Article Snippet: The E. coli strains MG1655, ATCC 8739, DH5α, and BL21(DE3) underwent genetic modification to enable ethylene glycol metabolism.

Techniques: Plasmid Preparation, Transformation Assay

The influence of TPA on the growth of ethylene glycol-utilizing E. coli chassis cells. A EG02; ( B ) EG-BL21(DE3). The E. coli strains, EG02 and EG-BL21(DE3), capable of utilizing ethylene glycol, were separately introduced into M9 medium containing varying concentrations of ethylene glycol, supplemented with equimolar doses of TPA-Na 2 (TPA). 1: 16 mM EG, 16 mM TPA; 2: 80 mM EG, 80 mM TPA; 3: 161 mM EG, 161 mM TPA; 4: 484 mM EG, 484 mM TPA; 5: 806 mM EG, 806 mM TPA; 6: 161 mM EG, 484 mM TPA. The red bars show the initial addition of ethylene glycol in M9 medium, with light red indicating the residual amounts after 72 h of fermentation. The green bars show the initial addition of TPA, with light green indicating the residual amounts after 72 h. The blue bars represent the OD 600 values of the strain after 72 h of fermentation. Error bars indicated standard error ( n = 3)

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock

doi: 10.1186/s13068-024-02568-4

Figure Lengend Snippet: The influence of TPA on the growth of ethylene glycol-utilizing E. coli chassis cells. A EG02; ( B ) EG-BL21(DE3). The E. coli strains, EG02 and EG-BL21(DE3), capable of utilizing ethylene glycol, were separately introduced into M9 medium containing varying concentrations of ethylene glycol, supplemented with equimolar doses of TPA-Na 2 (TPA). 1: 16 mM EG, 16 mM TPA; 2: 80 mM EG, 80 mM TPA; 3: 161 mM EG, 161 mM TPA; 4: 484 mM EG, 484 mM TPA; 5: 806 mM EG, 806 mM TPA; 6: 161 mM EG, 484 mM TPA. The red bars show the initial addition of ethylene glycol in M9 medium, with light red indicating the residual amounts after 72 h of fermentation. The green bars show the initial addition of TPA, with light green indicating the residual amounts after 72 h. The blue bars represent the OD 600 values of the strain after 72 h of fermentation. Error bars indicated standard error ( n = 3)

Article Snippet: The E. coli strains MG1655, ATCC 8739, DH5α, and BL21(DE3) underwent genetic modification to enable ethylene glycol metabolism.

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